Cai, Y. et al. We examined protein classes present in the hSNP regions by identifying 1,109 PantherDB matches, assigned to 26 protein classes from a combined set of all genes in hSNP dense regions (Supplementary Data 3). Biol. Here as example a link to the Map for Sensi Seeds Super Skunk. French, J. E. et al. Check it out and see what will happen if you click onto the Hybrids! Explore the rich history of cannabis, from its earliest recorded use to its current place in society. Initial sequencing and analysis of the human genome. AugustusPB transcripts that could not be confidently assigned to parent transcripts were discarded and not evaluated for novel contribution. From the pure landraces up Only coordinates in which at least one strain had a hSNP call were retained. In AugustusCGP the same types of evidence can be incorporated for either a subset or all species/ strains. 306, L10L22 (2014). Mouse genomic variation and its effect on phenotypes and gene regulation. Biol. The initial contigs were assembled from the paired-end sequencing reads using SGA v0.9.43 (see URLs)72. For each transMapped transcript alignment t, one way to identify its structure was a pipeline component we here refer to as AugustusTMR (TM=transMap, R=RNA-Seq). Each of the three M. m. domesticus strains (C67BL/6J, NOD/ShiLtJ, and WSB/EiJ) carries a different combination of Nlrp1 family members; Nlrp1d1f are novel strain-specific alleles that were previously unknown. You'll be able to concentrate on whatever it is that's important to you while remaining in an uplifted mood. 38, 21682171 (2006). Med. Anthony DiMeo. IFI16 DNA sensor is required for death of lymphoid CD4 T cells abortively infected with HIV. Gene retrotransposition has long been implicated in the creation of gene family diversity36, novel alleles conferring positively selected adaptations37. Mixing strains is best done with caution and a clear understanding of how each strain may affect you. carried out the genome analysis. Due to popularity, many cultivars of the same name have cropped up, leading to confusion and dispute over the original genetics and breeder. 9, 10991107 (2007). & Bui, J. D. Studies of the H60a locus in C57BL/6 and 129/Sv mouse strains identify the H60a 3UTR as a regulator of H60a expression. GENCODE: the reference human genome annotation for The ENCODE Project. D.T.O. Interestingly, within this region, Cpsf3 (21.29 Mb) is located on an island of high conservation in all strains and a homozygous C57BL/6NJ knockout produces subviable offspring35. Cannabis strains vary in their THC-to-CBD ratios, which can influence the overall effects. d, Representative sagittal brain images of matched controls (left) and Efcab3-like mutant (right), showing a larger cerebellum, enlarged lateral ventricle and increased size of the pontine nuclei (n=3, see Supplementary Fig. This process allows for the rescue of genes lost in the first round of filtering and consensus finding, as well as the discovery of polymorphic pseudogenes in the laboratory mouse lineage. 38, 240244 (2006). 5). Every sequencing lane was genotype checked against the mouse Hapmap SNP calls71 using the Samtools/Bcftools v1.1 'gtcheck' command. This makes it possible to exploit the combined evidence for gene finding and to discover genes that, for example, are only weakly expressed and partially supported in the reference strain but that have a high expression in other strains. One of the challenges of gene finders is to distinguish coding genes from pseudogenes and expressed non-coding genes that contain partial open reading frames. Learn about its many uses, its cultural significance, and its ongoing evolution as a natural medicine. This strain has also been speculated as Bob Marleys favorite. 7). Genet. Interestingly, the previously unannotated Efcab3-like gene is very close to the 17q21.31 syntenic region associated in humans to the Koolende Vries microdeletion syndrome (KdVS). Nat Genet 50, 15741583 (2018). Gene copy-number polymorphism caused by retrotransposition in humans. MeSH The combined SNP and indel error rate was 0.090.1 errors per kb, compared to 0.334 for MGSCv3 and 0.02 for GRCm38 (Supplementary Table 5). 2b and Supplementary Figs. 11 and Supplementary Data 7) and the near-complete inclusion of the Sts gene that was previously missing. The data about strains, products and their effects, contained on the website is based on the consumer reviews. D.J.A. Thank you for visiting nature.com. Shultz, L. D., Ishikawa, F. & Greiner, D. L. Humanized mice in translational biomedical research. These initial orthologs, along with strain-specific RNA-Seq (Supplementary Table 8), were input to AUGUSTUS74 one at a time to apply local strain-specific refinement. Correspondence to In addition to Aspen bedding substrate, standard environmental enrichment of a nestlet and a cardboard tunnel were provided. Mouse models of 17q21.31 microdeletion and microduplication syndromes highlight the importance of Kansl1 for cognition. Cannabis strains are generally classified into three main types: Indica, Sativa, and Hybrid. Members of group 4 Slfn genes50, Slfn8, Slfn9, and Slfn10, show significant sequence diversity among these strains. For each strain separately, coding genes from GENCODE M815 overlapping pass heterozygote dense windows were identified. 73, 196200 (2012). Consult with a cannabis healthcare professional for specific medical concerns, and always verify the legality of cannabis in your location. 17, 167 (2016). Genes related to immunological processes, particularly gene families involved in mediating innate immune responses (for example Raet1 and Nlrp1), exhibit great diversity among the strains, reflecting strain-specific disease associations, responses and susceptibility. Monroe, K. M. et al. Bustos, O. et al. This strain is an easy-to-grow photoperiod cultivar that will produce dense buds it's a great option for new growers. On average, 37 genes were putative novel loci (Supplementary Data 1) in wild-derived strains and 22 in classical strains. Additionally, an automated pseudogene annotation workflow, Pseudopipe25, alongside manually curated pseudogenes lifted over from the GRCm38 reference genome, identified an average of 11,000 (3,317 conserved between all strains) pseudogenes per strain (Supplementary Fig. Liebenauer, L. L. & Slotnick, B. M. Social organization and aggression in a group of olfactory bulbectomized male mice. Bethesda, MD 20894, Web Policies According to growers, this strain produces dark and light green foliage dotted with trichomes and burnt orange hairs. 35, 222236 (2015). Americo, J. L., Moss, B. This strain was produced when the growers crossed a female Triangle Kush plant with Animal Mints pollen. Environ. Ibeanusi V, Jeilani Y, Houston S, Doss D, Coley B. Biotechnol Lett. We used CRISPR (clustered regularly interspaced short palindromic repeats) to create Efcab3-like mutant mice (Efcab3em1(IMPC)Wtsi, see Methods) and recorded 188 primary phenotyping measures (Supplementary Data 9). I found that perfect headspace fifteen years ago with a strain dubbed Super Duper Silver Haze (really a bubblegum strain) that I used to score from PCC in Berkeley. b, Gene order across Raet1/H60 locus in the Collaborative Cross parental strains (A/J, NOD/ShiLtJ and 129S1/SvImJ share the same haplotype at this locus, represented by NOD/ShiLtJ). Thanks a lot for sharing your opinion! The largest difference in mean sequence divergence was between LTRs within and outside of hSNP dense regions. It will take 58 days to mature indoors. Ferris, M. T. et al. AskGrowers is an encyclopedia with cannabis-related data. We assessed the base accuracy of the strain chromosomes relative to two versions of the C57BL/6J reference genome (MGSCv311 and GRCm382) by first realigning all of the paired-end sequencing reads from each strain back to their respective genome assemblies, then using these alignments to identify SNPs and indels. strain SC2, which is the first methanotroph with confirmed high-affinity methane oxidation potential. Our data elucidated copy number variation previously unknown in mouse strain genomes and uncovered gene expansions, contractions, and novel alleles (<80% sequence identity). Leafy Mate is here to assist you in navigating the cannabis industry. Unauthorized use of these marks is strictly prohibited. is supported by R01 DK074656. Relying on the information from this website is strictly at your discretion. Creating reference gene annotation for the mouse C57BL6/J genome assembly. Kolmogorov, M. et al. Humboldt offers mostly heady, cerebral effects and a pleasantly floral flavor profile. Analysis of Genotype-Tissue Expression (GTEx) data62 in humans showed that the EFCAB13 locus is expressed across many tissue types, with the highest expression measured in testis and thyroid. 26, 342350 (2016). Both approaches resulted in the completion of ten new gene structures (for example, Supplementary Fig. It has been suggested that Nlrp1c may be the causal family member mediating resistance; Nlrp1c can be amplified from cDNA from PWK/PhJ macrophages but not CAST/EiJ54. Terpenes are aromatic compounds found in cannabis and other plants that contribute to each strain's unique scent and flavor profile. Chromosomal contacts connect loci associated with autism, BMI and head circumference phenotypes. We also created a de novo set of strain-specific genes and isoforms from Comparative Augustus (AugustusCGP)24 using the strain-specific RNA-Seq and the progressive Cactus alignment. Did you know that hemp can be used for more than just CBD oil? Both food and water were available ad libitum. M.K.S. Dickinson, M. E. et al. 116, Supplementary Tables 126 and Supplementary Note, Novel genes predicted in classical and wild-derived mouse strains, hSNP-dense regions in C57BL/6J and C57BL/6NJ and issues of GRCm38, Defense and immunity genes found in hSNP-dense regions, Assembly quality control of three immunity-related loci in WSB/EiJ, PWK/PhJ and CAST/EiJ, Reference corrections through strain assembly information, Phenotype of Efcab3-like/ mouse compared to control group, Definition of repeat classes and families. Genes involved in defense and immunity (the largest protein class represented by the combined gene set) were then retrieved and the strains that contributed genes to this protein class identified. These windows were then intersected with GENCODE M8 gene annotations; the total number of unique genes and base pair positions overlapping pass windows for each strain was calculated (Fig. The aim was to try to produce all splice forms from the reference (parent) genome that probably also exist in the target genome. Apart from having an amazing potency level, it has a classic O.G taste and a great flavor. 13). In the meantime, to ensure continued support, we are displaying the site without styles Genetics 206, 537556 (2017). A subsequent round of consensus finding was employed to incorporate these transcripts into the final consensus annotation set. It can induce euphoria, relaxation, and altered sensory perception. Approximately 90% of intergenic regions in the PWK/PhJ assembly of the Nlrp1 locus is composed of transposable elements (Fig. Cell Microbiol. Diversity Outbred mice identify population-based exposure thresholds and genetic factors that influence benzene-induced genotoxicity. Thereby, the evidence from t on the location of exons, introns and start and stop codons was given a much higher weight in order to produce the original splice form, also in cases where the majority of target RNA-Seq suggests a different major splice form. Bell, T. A. et al. Pairs of flanking guide RNAs (gRNAs) were designed using the WTSI Genome Editing (WGE) tool78 creating four gRNAs (two gRNAs 5 and two gRNAs 3 to the CE region, Supplementary Table 21). B., Steinfeldt, T. & Howard, J. C. Reciprocal virulence and resistance polymorphism in the relationship between Toxoplasma gondii and the house mouse. 2c). However, the most consistent reports maintain that a Cherry Pie/Burmese Thai cross makes Kirby, A. et al. Paten, B. et al. From its intriguing counterculture origins to its role in cannabis advocacy and legalization, explore how 420 became a global phenomenon. Many novel genes and transcripts were identified across all of the strains, highlighting unexplored sequence variation across the Mus lineage. We do not grow, sell or promote canna products nor propagate consumption or distribution of cannabis. Many paralogous gene families were represented among the hSNP regions (Supplementary Data 3), including genes with functional human orthologs. 16, 1927 (1997). Genet. This strain has become popular for its libido-boosting effects that will put you in the mood for romance. Despite this, previous SNP variation catalogs have identified high-quality heterozygous SNPs (hSNPs) when reads were aligned to the C57BL/6J reference genome12. In the genomic region around t, AUGUSTUS was set to predict a gene structure without alternative splicing, using evidence from t itself as well as from all RNA-Seq alignments in that region. Rapid heart rate? 10 feminised seedsActual special-offer onCannapot HanfshopNow 10.00 % reduced! Google Scholar. Some of the most potent terpenes are: In terms of smell and taste, it's pretty much exactly what you'd expect if you're familiar with the classic Headspace taste. designed and supervised the study. nov., a strictly anaerobic bacterium that grows via fermentation and reduces the cyclic nitramine explosive hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX). 7 and 8). The pontine nuclei were also increased in size by 42% (P=0.001) and the cerebellum by 27% (P=0.02); these two regions are involved in motor activity (Fig. These divergent profiles suggest that Nlrp1c is not the sole mediator of anthrax lethal toxin resistance in the mouse but several other members may be involved. Headspace potency is higher THC than average. Fixed chromatin was then digested with restriction enzyme Mbo I, the 5 overhangs were filled in with biotinylated nucleotides and then free blunt-ends were ligated. Manual annotation extended this gene to 188 exons (lower red track). Injected embryos were briefly cultured and oviductal embryo transfer performed in 0.5 days postcoital pseudopregnant female recipients (CBA/C57BL/6J). The Aspergillus-resistant locus 4 (Asprl4), one of several quantitative trait loci (QTLs) that mediate resistance against Aspergillus fumigatus infection, overlaps this locus and comprises of a 1 Mb (~10% of QTL) interval that, compared to other classical strains, contains a haplotype unique to NZO/HlLtJ (Supplementary Fig. Kwon MJ, Wei N, Millerick K, Popovic J, Finneran K. Curr Microbiol. Also, if you've been struggling with weight loss and don't know how to get out of the slump that comes with losing motivation to eat healthily, try this strain. USA 102, 814 (2005). The remaining candidate regions include large protein families (15/145, 10.3%) and repeat elements (17/145, 11.7%) (Supplementary Data 2). Codon-usage-based inhibition of HIV protein synthesis by human schlafen 11. Approximately 0.52% of total genome length per strain was unplaced and is composed of unknown gap bases (1849%) and repeat sequences (6179%) (Supplementary Table 2), with between 89 and 410 predicted genes per strain (Supplementary Table 3). Patierno, S. R. et al. At some loci, the genetic difference between the reference and sequenced strain genomes is comparable to that between humans and chimpanzees, making it hard to distinguish whether a read is mismapped or highly divergent. Metabolism of hexahydro-1,3,5-trinitro-1,3,5-triazine through initial reduction to hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine followed by denitration in Clostridium bifermentans HAW-1. Histograms showing the neuroanatomical features as percentage increase or decrease of the assessed brain regions in Efcab3-like mutant mice compared to matched controls (right). Publishers note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Same-day pickup or delivery in Portsmouth, ENG. After ligation, cross-links were reversed and the DNA was purified from protein. Corresponding brain regions are labelled with a number that is described below the panel (Supplementary Table 15). Use the Previous and Next buttons to navigate the slides or the slide controller buttons at the end to navigate through each slide. For LTRs, LINEs and short interspersed nuclear elements (SINEs), the mean percentage sequence divergence was significantly lower (P<11022) within hSNP regions compared to the rest of the genome (Fig. We are here to help! 1c) and are over-represented with genes associated with immunity, sensory, sexual reproduction and behavioral phenotypes (Fig. Host genetic diversity enables Ebola hemorrhagic fever pathogenesis and resistance. It's also important to start with a low dose and gradually increase as needed, especially if you're new to cannabis. This collection comprises a large and diverse array of laboratory strains, including those closely related to commonly used mouse cell lines (BALB/3T3 and L929, derived from BALB/c and C3H related strains, respectively), embryonic stem cell-derived gene knockouts (historically 129 related strains)14, mouse models of human disease (such as NOD-related nude mice)15, gene knockout background strains (C57BL/6NJ)16, the founders of commonly used recombinant inbred lines such as the AKXD, BXA, BXD, CXB, and CC17, and outbred mapping populations such as the DO and the heterogeneous stock18. Receive updates on new products, special offers, and industry news. WebAfter cultivation with 21% (v/v) O2 in the headspace, no recombinant hydrogenase activity was observed in soluble extracts of wild-type BL21, although low levels of activity could be 10). Medical patients choose Head space to relieve symptoms of stress, pain Newly annotated members Nlrp1b2 and Nlrp1d appear functionally intact in CAST/EiJ but were both predicted as pseudogenes in PWK/PhJ due to the presence of stop codons or frameshift mutations. Sixteen diverse laboratory mouse reference genomes define strain-specific haplotypes and novel functional loci, https://doi.org/10.1038/s41588-018-0223-8. Colors represent the phylogenetic relationships (top, amino acid neighbor joining tree of NBD domain) and the relative gene order across strains (bottom). Epub 2016 Nov 17. Population variation in NAIP functional copy number confers increased cell death upon Legionella pneumophila infection. 2017 Apr;6(2):e00423. Sci. Arbogast, T. et al. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Cell Tissue Res. 12). To obtain For the latter, CDS and intron hints were generated from the GENCODE VM8 protein-coding gene set for the reference strain. Performed phytochemical study contributes to the knowledge of volatile organic compounds (VOCs) of Halopteris filicina (Grateloup) Ktzing, Dictyota dichotoma (Hudson) If multiple assignments were created, attempts to resolve them were made by finding if any gene had a Jaccard distance 0.2 greater than any other; otherwise, they were discarded. & Mott, R. Applying mouse complex-trait resources to behavioural genetics. Sequence accessions for the three immune-related loci on chromosome 11 are available from the European Nucleotide Archive (Supplementary Table 23). and transmitted securely. Stanke, M., Diekhans, M., Baertsch, R. & Haussler, D. Using native and syntenically mapped cDNA alignments to improve de novo gene finding. The strains mysterious background hasnt stopped it from becoming popular around the world, though. 2020 Sep 15;5(5):e00277-20. A cross between Purple Punch and Clementine, Mimosa is a sativa-dominant hybrid strain. White coloring indicates a P value>0.05 and grey indicates that the brain region could not be confidently tested due to missing data. Be mindful of the method of consumption (e.g., smoking, vaping, edibles) as it can impact the onset and duration of effects. First, these loci were compared to the respective regions in the C57BL/6NJ assembly and used to identify contigs from public assemblies of the reference strain previously omitted due to insufficient overlap. In the house mouse, Nlrp1 alleles are involved in sensing Bacillus anthracis lethal toxin, leading to inflammasome activation and pyroptosis of macrophages52,53. 7, e1000112 (2009). It helps stimulate an appetite in people who have trouble eating due to stress or depression. 1, 119126 (2000). Genet. The RNA-Seq hints were generated for each of the novel strains by aligning RNA-Seq reads to the native genome with the spliced aligner STAR16. All rights reserved. Rev. Further information on research design is available in the Nature Research Reporting Summary linked to this article. 8, 259268 (2008). volume50,pages 15741583 (2018)Cite this article. See the Mouse Genomes Annotation pipeline documentation for details on this process (see URLs). Fine mapping in 94 inbred mouse strains using a high-density haplotype resource. The consensus gene sets were subsetted into a basic gene set following the methodology used by GENCODE60. The assembly and alignment of a variety of haplotypes at loci heterogenous amongst the laboratory strains allows for analysis of regions previously not placed in the reference assembly. Webheadspace strain geneticsmegabus cardiff to london. (2017). J. Virol. Would you like email updates of new search results? PubMed Central The separations of Chicago read pairs mapped within draft scaffolds were analyzed by HiRise to produce a likelihood model for genomic distance between read pairs. WebSeedFinders Cannabis-Genetic-Search Origin and genetic characteristics Here you can look for Cannabis Strains who have certain genetic characteristics. Grey indicates the strain base agrees with the reference, other colors indicate SNP differences and height corresponds to sequencing depth. This is consistent with the promoter of the full-length gene being present upstream from the EFCAB13 version, which is supported by H3K4me3 analysis (Supplementary Fig. Dummer, P. D. et al. Examining only repeat elements with less than 1% divergence, we found these regions are significantly enriched for LTRs (empirical P<1107) and LINEs (empirical P=0.047). 1e). WebBred by Liberty Reach, this strain is tasty like the classic GSC with a sweet profile thats also crisp and fresh. Churchill, G. A. et al. WebIts easy to grow even if this is your first-ever strain. A co-evolutionary arms race: trypanosomes shaping the human genome, humans shaping the trypanosome genome. The care and use of mice in the study was carried out in accordance with UK Home Office regulations, UK Animals (Scientific Procedures) Act of 1986 under a UK Home Office license that approved this work, which was reviewed regularly by the WTSI Animal Welfare and Ethical Review Body. 60, 403409 (1996). Genome 26, 366378 (2015). We discovered novel gene members at several important immune loci regulating innate and adaptive responses to infection. The generation and assembly of a reference genome for C57BL/6J accelerated the discovery of the genetic landscape underlying phenotypic variation11. Putnam, N. H. et al. We're still gathering information about the Headspace strain. Gene sets for each strain were then combined and, using PantherDB76, were classified based on protein class annotations (Fig. In mouse, embryonic death may occur between strains carrying incompatible Slfn haplotypes59. The genome structure of the Nlrp1 locus in PWK/PhJ, CAST/EiJ, WSB/EiJ, and NOD/ShiLtJ was confirmed using Fiber-FISH (Supplementary Fig. Prostate Cancer 1, 118124 (2002). All mice were maintained in a specific pathogen-free facility with sentinel monitoring at standard temperature (1923C) and humidity (55% 10%), on a 12 h dark, 12 h light cycle (lights on 7:3019:00) and fed a standard rodent chow (LabDiet 50213, 9% crude fat content, 21% kcal as fat, 0.276 ppm cholesterol). Headspace has a modest amount of 19% THC on average although this number could vary from one grower to another. EMPLOYMENT 16-19: Indiana University; EMPLOYMENT 14-15: University of California. Notably, 89 immune-related genes were identified in classical strains, 84 of which were shared with at least one of the wild-derived strains (Fig. Retrotransposition of gene transcripts leads to structural variation in mammalian genomes. Genome Biol. 2014 Jun;68(6):743-50. doi: 10.1007/s00284-014-0531-x. Mus is the first mammalian lineage to have multiple chromosome-scale genomes. High will clog probes. 1, https://doi.org/10.2478/mngs-2013-0001 (2013). Mitochondrial genome (mtDNA) assemblies for 14 strains supported previously published sequences20, although a small number of high-quality novel sequence variants in AKR/J, BALB/cJ, C3H/HeJ, and LP/J conflicted with GenBank entries (Supplementary Table 4). The Mroh3 gene, which was originally annotated as an unprocessed pseudogene, was updated to a protein-coding gene due to the identification of a novel intron that permitted extension of the CDS to full length. After scaffolding, shotgun sequences were used to close gaps between contigs. https://doi.org/10.1371/journal.pgen.1006886 (2017). ISSN 1061-4036 (print). We used the C57BL/6J GRCm38 sequence as a single reference and found that 95% adjacent synteny block pairs from the assemblies were also adjacent in C57BL/6J reference. Simpson, E. M. et al. In contrast, the EFCAB3 locus only has low-level measurable expression in testis. It can give off an earthy, lemony, pungent, spicy, or dank aroma. Transcriptional activity and strain-specific history of mouse pseudogenes, Inbred lab mice are not isogenic: genetic variation within inbred strains used to infer the mutation rate per nucleotide site, Extended regions of suspected mis-assembly in the rat reference genome, Towards a reference genome that captures global genetic diversity, Population structure and inbreeding in wild house mice (Mus musculus) at different geographic scales, Towards complete and error-free genome assemblies of all vertebrate species, The sequences of 150,119 genomes in the UK Biobank, A unified catalog of 204,938 reference genomes from the human gut microbiome, https://github.com/ucsc-mus-strain-cactus/MouseGenomesAnnotationPipeline, https://doi.org/10.1371/journal.pgen.1006886, https://www.biorxiv.org/content/early/2018/02/11/088435, http://creativecommons.org/licenses/by/4.0/, Analysis of structural variation among inbred mouse strains, Multiple genome alignment in the telomere-to-telomere assembly era, Genetic quality: a complex issue for experimental study reproducibility, MoG+: a database of genomic variations across three mouse subspecies for biomedical research, Sign up for Nature Briefing: Translational Research. Immunol. Strain-specific haplotype associations with Asprl4 and survival have been reported for CAST/EiJ and NZO/HlLtJ, both of which exhibit resistance to A. fumigatus infection47 and they are also the only strains to have lost H60 alleles at this locus. Illumina sequencing compatible Mate Pair libraries were created at 3 and 6 kb according to the Sanger method70. c, Novel protein-coding alleles of the Nlrp1 gene family in the wild-derived strains and two classical inbred strains. The model was used to identify putative misjoins and score prospective joins. Capewell, P., Cooper, A., Clucas, C., Weir, W. & Macleod, A. 6a). This work was supported by the Medical Research Council (MR/L007428/1), BBSRC (BB/M000281/1), and the Wellcome Trust. Neuroanatomical studies were performed blind with experimenters not knowing the genotype of the mouse, on three 16-week-old matched control male mice in C57BL/6N background and three 16-week-old homozygous knockout of Efcab3.
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